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Fly Outcrossing
Author: Jennifer Beck, updated date: , view: 11, Q&A: 0
Tags: Fly, Drosophila and Outcross

Procedure

  1. w-; p[w+, transgene]/p[w+, transgene] ☿☿ x w-; +/+ ♂♂ (w1118 original)
    ↓ select red eye

  2. w-; p[w+, transgene]/+ ☿☿ x w-; +/+ ♂♂ (w1118 original)
    ↓ select red eye

  3. w-; p[w+, transgene]/+ ☿☿ x w-; +/+ ♂♂ (w1118 original)
    ↓ select red eye

  4. w-; p[w+, transgene]/+ ☿☿ x w-; +/+ ♂♂ (w1118 original)
    ↓ select red eye

  5. w-; p[w+, transgene]/+ ☿☿ x w-; +/+ ♂♂ (w1118 original)
    ↓ select red eye

  6. w-; p[w+, transgene]/+ ☿☿ x w-; CyO/Sco or TM3, Sb/TM6B, Sb ♂♂ (w1118 outcrossed)
    ↓ 

  7. w-; p[w+, transgene]/CyO or TM3, Sb ☿☿ x w-; CyO/Sco or TM3, Sb/TM6B, Sb ♂♂ (w1118 outcrossed)
    ↓ 

  8. w-; p[w+, transgene]/CyO or TM3, Sb ☿☿ x w-; p[w+, transgene]/CyO or TM3, Sb ♂♂ (☿ and ♂ = same genotype)
    ↓ 

  9. w-; p[w+, transgene]/p[w+, transgene] ☿☿ x w-; p[w+, transgene]/p[w+, transgene] ♂♂ (☿ and ♂ = same genotype)

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