×
Please leave your comments or questions
 
Pseudomonas Aeruginosa PA14 Infection Assay - C. elegans
Author: Harshi Peiris, updated date: , view: 3, Q&A: 0
Tags: Worm and Infection

Procedure

Streak Pseudomonas aeruginosa PA14 on to a plate of Brain heart fusion. This plate can be maintained for ~14 days. Stream fresh if the plate is more than 14 days old.


Grow the bacteria in Brain heart fusion broth overnight with no antibiotics.


Plate the bacteria on fresh NGM plates. Make sure to spread the bacteria throughout the plate (worms tend to evade PA-14, so don't leave empty spaces within the plate)


Grow the bacteria for ~24 hours. If the bacteria grows, the plate will look green in color.


Transfer worms on to the plate for day 1 (with fudr) 


Transfer worms to a fresh plate on day 2 (with fudr)


Transfer worms to a fresh plate on day 3 (with fudr)


Transfer worms to a fresh plate on day 5 (with fudr)


Transfer worms every other day until the assay/lifespan ends

Comment
Please login to post your questions/comments. Your questions will be directed to the authors of the protocol. The authors will be requested to answer your questions at their earliest convenience. Once your questions are answered, you will be informed using the email address that you register with bio-protocol.
You are highly recommended to post your data including images for the troubleshooting.
You are highly recommended to post your data (images or even videos) for the troubleshooting. For uploading videos, you may need a Google account because Bio-protocol uses YouTube to host videos.