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Response to the request
Last updated date: Apr 3, 2021 Views: 763 Forks: 0
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Hi,
We're glad that you're interested in our method, TRACE-seq. The detail protocol of TRACE-seq has been uploaded to the method section "TRACE-seq library preparation and sequencing" of the online version of our paper.
As for nucleic acid isolation, total RNA was extracted from cells with TRIzol (Invitrogen), according to the manufacturer’s instructions (https://www.thermofisher.com/order/catalog/product/15596018?SID=srch-srp-15596018#/15596018?SID=srch-srp-15596018). For mRNA isolation, two successive rounds of poly(A)+ selection were performed using oligo(dT)25 dynabeads (Invitrogen), according to the manufacturer's instructions (https://www.thermofisher.com/order/catalog/product/61005?SID=srch-srp-61005#/61005?SID=srch-srp-61005). We believe that the detailed user guidelines in the websites will help a lot.
Please feel free to let us know if you have any question or need any additional information.
- Lu, B and Yi, C(2021). Response to the request. Bio-protocol Preprint. bio-protocol.org/prep994.
- Lu, B., Dong, L., Yi, D., Zhang, M., Zhu, C., Li, X. and Yi, C.(2020). Transposase-assisted tagmentation of RNA/DNA hybrid duplexes. eLife. DOI: 10.7554/eLife.54919
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