2.4. Growth Curves

In order to assess whether QAL exerted a partial inhibitory effect at sub-MIC concentrations, growth curve analysis was performed. Overnight cultures of P. aeruginosa strains were diluted 1:100 in fresh LB broth both in the absence and presence of QAL at 0.31 and 0.62 mg/mL and incubated at 37 °C in an orbital shaker. The growth curves were then determined by measuring the OD at 570 nm at 1 h time intervals up to 10 h and at 24 h.