All codes and commands used to process the data and generate all plots and figures are available at GitHub: https://github.com/Terkild/CITE-seq_optimization (Buus, 2021; copy archived at swh:1:rev:1c7fcabb18a1971dc4d6e29bc3ed4f6f36b2361f).
UMI count matrices from the optimization experiment have been deposited at FigShare with DOI: https://doi.org/10.6084/m9.figshare.c.5018987. The feature barcode 3′ and 5′ VDJ 10X datasets are available from the 10X Genomics website.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.