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Cell growth assay

XW Xiaowen Wang
HZ Hong Zhang
RS Russell Sapio
JY Jun Yang
JW Justin Wong
XZ Xin Zhang
JG Jessie Y. Guo
SP Sharon Pine
HR Holly Van Remmen
HL Hong Li
EW Eileen White
CL Chen Liu
MK Megerditch Kiledjian
DP Dimitri G. Pestov
XZ X. F. Steven Zheng
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Cell growth was measured by a sulforhodamine B (SRB) colorimetric assay54. Cells were seeded in triplicate at a predetermined density (500–2000 cells per well) in 96-well plates. Cells were fixed in place with 100 μl 10% trichloroacetic acid (Sigma, T6399) at 4 °C for 1 h. After washing and air drying, cells were then stained with 50 μl of 0.057% (wt/vol) SRB (Sigma, S1402, dissolved in 1% acetic acid) for 30 min. Unbound dye was washed away once with water and three times in 1% acetic acid. The cells were allowed to air dry before the dye was solubilized by adding 200 μl of 10 mM Tris base solution (pH 10.5). Conversion of colored end product was measured by optical density at 510 nm in a TECAN Infinite M200PRO plate reader.

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