4.1. Identification of TRP Genes in C. gigas

To identify the full set of TRP genes in C. gigas, we downloaded the TRP protein sequences from several representative invertebrates, including Branchiostoma belcheri, Ciona intestinalis, Acanthaster planci, Strongylocentrotus purpuratus, Drosophila melanogaster, Bombyx mori, Crassostrea virginica, Mizuhopecten yessoensis, Caenorhabditis elegans, and Exaiptasia pallida, and several representative vertebrates, including Homo sapiens, Mus musculus, Bos taurus, Meleagris gallopavo, Gallus gallus, Chrysemys picta bellii, Anolis carolinensis, Xenopus laevis, Xenopus tropicalis, Pangasianodon hypophthalmus, and Danio rerio. The detailed information of these TRPs were provided in Supplementary Table S3. These sequences were downloaded from NCBI (https://www.ncbi.nlm.nih.gov, accessed on 3 March 2021) and Ensembl (http://grch37.ensembl.org/index.html, accessed on 3 March 2021) databases and used as queries to search against the reference genome C. gigas in the NCBI (Assembly: cgigas_uk_roslin_v1, with accession of GCA_902806645.1) using BLAST program with the E-value of 1E-10. The identified TRP-related sequences were further manually curated, followed by functional domain analysis using Conserved domain Search program (https://www.ncbi.nlm.nih.gov/Structure/cdd/wrpsb.cgi, accessed on 3 March 2021). The identifies of predicted TRP proteins from C. gigas were further confirmed by protein BLAST (BLASTP) [48] against NCBI non-redundant (Nr) protein sequence database. The distribution of the identified TRP genes on the chromosomes were drawn using TBtools [49].