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Quantitative PCR

PG Priyatansh Gurha
XC Xiaofan Chen
RL Raffaella Lombardi
JW James T. Willerson
AM AJ Marian
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Total RNA (including miRNA) was extracted and reverse transcribed. MiRNAs were amplified using specific TaqMan gene expression assays and TaqMan MicroRNA assays (Online Table I). Gapdh and snoRNA202 transcript levels were used for normalization for mRNA and miRNA levels, respectively. 2ΔΔCt method was used to calculate the normalized gene expression values.

Copyright and License information:  ©2016-07-28

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