Mouse ACBD7 mRNA sequencing

Mouse hypothalamic mRNA was purified using RNeasy Mini Kit (Qiagen, Toronto, Canada, ON), according to the manufacturer's instructions. The RNA concentrations were estimated from absorbance at 260 nm. cDNA synthesis was performed using random hexamer primers and the expand reverse transcriptase (Roche Diagnostics, Laval, Canada, QC). The Acbd7 cDNA was amplified using primers specific to the Acbd7 sequence (Acbd7-FP: 5’-TCCGTGTCTCATCATTATGTCC-3’; Acbd7-RP: 5’-AGGTAACCATGCTGACAGTCCT-3’) designed to cover the entire open reading frame of the Acbd7 mRNA (410-bp). After purification on agarose gel, PRC product was sequenced by the molecular platform of Laval University (Quebec City, Canada, QC).