Bioinformatics methods.

TB Thierry Bardin
AD Asen Daskalov
SB Sophie Barrouilhet
AG Alexandra Granger-Farbos
BS Bénédicte Salin
CB Corinne Blancard
BK Brice Kauffmann
SS Sven J. Saupe
VC Virginie Coustou
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BLAST analyses on the P. anserina genome were achieved on the Podospora anserina Genome Project site (podospora.i2bc.paris-saclay.fr/). Sequence alignments were performed with Clustal Omega or MAFFT (www.ebi.ac.uk) and edited with Jalview (www.jalview.org/). To generate the hidden Markov model (HMM) profile signatures for PP and RHIM, PSI-BLAST searches were performed (blast.ncbi.nlm.nih.gov) using the HELLP PP sequence and the RHIMs of human RIP3, matching sequences were aligned with Clustal Omega, and the alignment was analyzed with Skylign (skylign.org). Secondary structure predictions were performed with PSIPRED (bioinf.cs.ucl.ac.uk/psipred/). Hidden Markov model searches were performed using HHPred (toolkit.tuebingen.mpg.de) and Jackhhmer (www.ebi.ac.uk/Tools/hmmer/search/phmmer), both with default settings. The prediction of transmembrane helix was performed with the TMHMM server (www.cbs.dtu.dk/services/TMHMM).

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