Cryo-sections from cell implant studies were detected for tissue non-specific alkaline phosphatase (ALP; 1:300, ab65834, Abcam) and the nuclei were co-stained with DAPI. Imaging was performed using a Nikon Eclipse 90i Upright Fluorescence Microscope.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.