2.1. Animals

The recent experiment was conducted in the period of October 2018 to October 2019 in Egypt, in the Faculty of Science at Beni-Suef University. It included 50 Wistar rats of male gender (120–150 g in weight; 10–12 weeks old). Rats were bought from Egyptian Organization for Biological Products and Vaccines (VACSERA), Helwan Station, Cairo, Egypt. The animals were overseen for two weeks before the starting of the experiment to exclude any intercurrent infection. Rats were accommodated in calibrated cages of polypropylene and kept in controlled circumstances with a humidity around (55 ± 5%); also, room temperature of (22 ± 2°C), and 12-hour lighting and 12-hour darkness cycle. Animals were given drinking water and fed rat chow diet ad libitum. The experimental animals used in the current research were treated in accordance with the Canadian Council's Principals and Guidelines of Animal Care and Experimental Animal Ethics Regional Committee, Faculty of Science, University of Beni-Suef, Egypt, which approved the experimental work. All attempts were made to minimize the number of struggling animals during the study as possible.