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Flow cytometry

XW Xiaonan Wang
CP Carlotta Peticone
EK Ekaterini Kotsopoulou
BG Berthold Göttgens
FC Fernando J Calero-Nieto
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Cellular composition of leukapheresis samples was determined by flow cytometry using the following antibodies (20 min at 4°C): CD3 (BD), CD19 (BioLegend), and CD56 (BioLegend).

Expression of exhaustion markers was determined by flow cytometry using the following antibodies (20 min at 4°C): CD3 (BD), RQR8 (Qbend10 antibody; R&D), CD8 (BioLegend), LAG3 (Enzo Life Sciences), PD1 (BioLegend), and TIM3 (BioLegend).

All samples were counterstained with 7AAD (BioLegend) to exclude dead cells.

BD Celesta was used for cell acquisition, and data were analyzed using FlowJo V10 (Treestar). Representative plots showing the gating strategy are shown in Supplementary Figure 6A.

Copyright and License information:  ©2021 The Author(s). Published with license by Taylor & Francis Group, LLC.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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