2.5. Quantitative RT-PCR

Total RNA was extracted using the TRIzol reagent and made into cDNA using reverse transcription kit. Then, quantitative PCR was performed using the SYBR MasterMix (4913850001, Roche, Basel, Switzerland), ABI 7900HT, according to the manufacturer's instructions, and then the difference was calculated using the 2^–ΔΔCT. KIF11 primer is as follows: forward 5′-GAA CAATCATTAGCAGCAGAA-3′ and reverse 5′-TCAGTATAGACA CCACAGTTG-3′ [27]. β-Actin primer is as follows: forward primer 5′-TAATCTTCGCCTTAA TACTT-3′ and reverse primer 5′-AGCCTTCATACATCTCAA-3′ [27].