For GSE106899, based on the logCPM values, the DEGs between COPD and COPD with NSCLC were identified by the edgeR package. For GSE12472 and GSE12428, the classical Bayesian method provided by the limma package was used to analyze the DEGs 20 (Version 3.10.3, http://www.bioconductor.org/packages/2.9/bioc/html/limma.html). The Benjamini & Hochberg method was used to perform multiple test calibration on the p.value and logFC values of the above genes to obtain the corrected p value (adj.P.Value). The adj.P.Val<0.05 and |logFC|>0.585 were selected as the threshold for the following analysis.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.