Mice

Female C57BL/6 mice (18–22 g, 6–8 weeks, n = 115) were purchased from Beijing Vital River Laboratory Animal Technology Co., Ltd (Beijing, China) and were acclimated to the laboratory housing for 3 to 4 days. Mice were housed in a cage of three in specific-pathogen-free (SPF) conditions with temperature of 18–22°C, humidity of 40–60% and a 12:12 light/dark cycle. All animals had free access to sterile food and water. Animals were monitored daily for health status and weighed once a week. Procedures were performed by experienced operators and involved only brief restraint. After injection procedures, animals were closely monitored for signs of pain or distress, motility and level of food consumption. Animals that reached the humane endpoints will be euthanized. No death or adverse effect was observed in mice during the study. At the end of experiments, mice are euthanized by cervical dislocation. The researchers were blinded with regards to pharmacological treatment while processing data. All experiments were approved by the Institutional Animal Treatment and Use Committee of Institute of Basic Research in Clinical Medicine, China Academy of Chinese Medica Sciences (code: 20190901058) and conducted in accordance with Chinese National Guidelines (GB/T 35892–20181). The ARRIVE Essential 10 checklist were included in S1 Checklist.