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Preprocessing of scRNA-seq data

CB Christine Bangert
KR Katharina Rindler
TK Thomas Krausgruber
NA Natalia Alkon
FT Felix M. Thaler
HK Harald Kurz
TA Tanya Ayub
DD Denis Demirtas
NF Nikolaus Fortelny
VV Vera Vorstandlechner
WB Wolfgang M. Bauer
TQ Tamara Quint
MM Michael Mildner
CJ Constanze Jonak
AE Adelheid Elbe-Bürger
JG Johannes Griss
CB Christoph Bock
PB Patrick M. Brunner
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Preprocessing of the scRNA-seq data was performed using Cell Ranger version 3.0.2 (10x Genomics). Raw sequencing files were demultiplexed using the Cell Ranger command “mkfastq.” Each sample was aligned to the human reference genome assembly “refdata-cellranger-GRCh38-3.0.0” using the Cell Ranger command “count.” Raw expression data were then loaded into R version 3.5.1 (2018-07-02).

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