Quantitative PCR

RNA was isolated from sorted or cultured ILC2s using the RNeasy Micro Kit (Qiagen) according to the manufacturer’s instructions. RNA was synthesized into complementary DNA using RevertAid H Minus Reverse Transcriptase and random hexamer primers in the presence of RiboLock RNase inhibitor (Thermo Fisher Scientific). For quantitative reverse transcription polymerase chain reaction (qRT-PCR) reactions, probes from the Universal ProbeLibrary Set (Roche Applied Science) and TaqMan Universal Master Mix were used (Applied Biosystems, Foster City, CA, USA). qRT-PCRs were performed using the Prism 7300 Sequence Detection System (Applied Biosystems).