Study design

The objectives of this study were to determine whether IL-33 served a distinct biological role in different cell types and to investigate the mechanism of IL-33 delivery from myeloid cells. Experiments using mice aimed for group sizes of four to eight mice (matched for age and sex) and were repeated two to three times to assure reproducibility. Mice were tattooed for identification, with experimental groups randomized across cages to account for any microisolator effects. Parasite infection experiments were mostly done with male mice on the basis of previous experience, whereas cell culture experiments used tissues from both sexes in equal proportion for all experiments. To address subjectivity during the study, all animal cages and experimental samples/groups were assigned a letter number code to ensure that experiments were conducted in a blinded manner.

Justification for removal of any outliers was solely based on Grubb’s test. For all flow cytometry experiments, we included negative controls, such as fluorescence minus one controls, to establish reliable and reproducible gates for each marker.