Mice and infections

C57BL/6 (CD45.2), B6.CH-2^bm1 (bm1), OT-I.CD45.1, OT-II.CD45.1, CCR2 KO, and BALB/c mice were bred in-house and housed in specific pathogen–free conditions in the animal facility at the Doherty Institute of Infection and Immunity, the University of Melbourne. All experiments were done in accordance with the Institutional Animal Care and Use Committee guidelines of the University of Melbourne. Mice were infected intranasally in the URT with 10⁴ plaque-forming units (PFU) of x31-OVA₁ (encodes the OVA_257–264 epitope within the neuraminidase stalk) (7) or 10⁴ PFU of x31-OVA₂ (encodes the OVA_323–339 epitope within the neuraminidase stalk) (8) in a volume of 10 μl. In some experiments, mice received an URT infection with 10⁵ PFU of PR8-LAIV, which was generated as described (37). Mice were infected intranasally with 10⁸ CFU of S. aureus [JKD6159, CA-MRSA (ST93) (38)] or S. aureus (Newman, MSSA CC8) (39) (40) in a volume of 10 μl as previously described (41).