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RNA isolation and RT-qPCR
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Cells were harvested in TRIzol before purification via Direct-zol RNA MiniPrep (Genesee Scientific) as per the manufacturer’s instructions with an additional dry spin after disposing of the final wash to prevent carryover. Complementary DNA (cDNA) was generated using EcoDry double primed premix (Clontech). qPCR was performed using iTaq supermix on the Bio-Rad CFX96 Real-Time System. PCR primer sequences are listed in the Supplementary Materials.
Copyright and License information: ©2020
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