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Rats were killed under general anaesthesia at the end of the 6th week, and bilateral femoral heads were obtained and fixed in 4% paraformaldehyde. All samples were scanned with a 9‐micron voxel size micro‐CT scanner (Skyscan 1176). The acquisition conditions are 35 kV of energy and 220 mA of intensity. The images were managed by CTVol software and reconstructed. Parameters, including bone mineral density (BMD), trabecular thickness (Tb.Th), trabecular number (Tb.N) and trabecular bone volume fraction (BV/TV), were calculated from the reconstructed images.
Copyright and License information: ©2020 The Authors. published by Foundation for Cellular and Molecular Medicine and John Wiley & Sons Ltd
This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
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