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Whole mount in situ hybridization was performed as described previously [34] with a hybridization temperature of 65°C. Probes were transcribed from linearized plasmids using T7 RNA polymerase: Etv5 (Xin Sun/ Gail Martin), Ptc1 [35], Msx1 [36], FGF8 [37], and Sox10 [38]
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