Flow cytometry for cell surface antigen expression

WL Wen-Chieh Liao
CL Chih-Kai Liao
TT To-Jung Tseng
YH Ying-Jui Ho
YC Ying-Ru Chen
KL Kuan-Hung Lin
TL Te-Jen Lai
CL Chyn-Tair Lan
KW Kuo-Chen Wei
CL Chiung-Hui Liu
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GBM cells were detached with 10 mM EDTA and stained with CS56 antibody at 1∶100 dilutions on ice for 30 min. Cells were incubated with fluorescein isothiocyanate (FITC)-conjugated anti-mouse IgM antibody on ice for 30 min. For measuring cell surface PDGFRA expression, cycloheximide-treated cells were detached and immediately fixed with 4% paraformaldehyde for 15 min. Cells were stained with PDGFRA antibody at 1∶200 dilutions. A FITC-conjugated anti-rabbit IgG was used as the secondary antibody. Florescence intensity was analyzed by FACScan cytometer (BD Pharmingen).

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