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1 x 105 alveolar macrophages isolated from the lungs of naive, and post-influenza mice, were plated on a white 96-well flat-bottom plate in 100μl and allowed to settle for 1 hour at room temperature 50μM of luminol (Sigma) and 1.2U/ml Horseradish Peroxidase (Sigma) were added with 15μl of TIGR4 (1 x 104 CFU). The plate was spun to collect stimulus and luminescence was immediately read on a Safire II plate reader (Tecan).
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