Statistical Analysis

Data acquisition and analysis were done without knowledge of group identity. Group sizes were determined by power calculations. We used only male mice, because aged female mice were not available from the vendor. The hypothesis was that Ucn2 gene transfer would effectively treat LV diastolic dysfunction in aged mice. First, young versus aged mice that received intravenous saline were compared to establish that LV diastolic dysfunction was present in aged mice. Subsequently, aged mice that received Ucn2 gene transfer versus saline were compared to determine whether Ucn2 gene transfer in aged mice was associated with increased LV diastolic function. To test for group differences, Student’s t test (unpaired, two-tailed) was used. Bonferroni correction was used for multiple testing. Analyses were performed using GraphPad Prism v6.07 (GraphPad Software, San Diego, CA, USA). The null hypothesis was rejected when p < 0.05.