2.2. Tissue Preparation

Animals were sacrificed by isoflurane at the end of the 12 month treatment regimen. Brains were promptly removed and immersed in 4% paraformaldehyde for 24 h then transferred to a 30% sucrose solution and stored for later histological analysis. Brain sectioning and amyloid imaging was not performed on 3 SED, 4 LOW, and 2 HI mice due to tissue damage during the processing procedure. The soleus muscle was removed from each hindlimb, snap frozen on dry ice, and stored at −80 °C for citrate synthase analysis.