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At 48 h after transfection with T-HSV-TK-EGFP-SV40, cells were collected and washed with phosphate-buffered saline. To test the expression levels of EGFP, the cells were subjected to flow cytometry analysis using a CytoFLEX flow cytometry (Beckman Coulter, Brea, CA, USA). HEK 293FT-CXX-4 cells were subjected to flow cytometry analysis at 48 h after treatment with Apicidin or M-344.
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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
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