Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

Light microscopy

TA Tommi Anttonen
AH Anni Herranen
JV Jussi Virkkala
AK Anna Kirjavainen
PE Pinja Elomaa
ML Maarja Laos
XL Xingqun Liang
JY Jukka Ylikoski
AB Axel Behrens
UP Ulla Pirvola
request Request a Protocol
ask Ask a question
Favorite

Sections were analyzed with bright-field and differential interference contrast (DIC) optics under BX61 Microscope equipped with UPlanApo 10×, 20×, and 60× objectives. Images were acquired through DP73 CCD Color Camera and CellSens Software (all from Olympus). Confocal images were acquired using a Leica TCS SP5 laser-scanning microscope with Plan Apochromat 10×/0.40 numerical aperture (NA) and 63×/1.3 NA glycerol objectives. The acquisition software was Leica LAS AF. ImageJ was used for the creation of z-projections.

Copyright and License information:  ©2016 Anttonen et al.
This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A