Protein purification

Katanin (6xHis-MEI-1 WT or variant Strep-MEI-2) were purified from BL21 (DE3) bacteria transformed with pNJ plasmids (see Table S1) as described in Joly et al. (2016). Before storage, buffer was exchanged to 50 mM Tris HCl, pH 8.0, 500 mM NaCl, and 5% glycerol using G25 columns, and the proteins were snap frozen in liquid nitrogen and stored at −80°C.

6xHis-MEI-1 and Strep-MEI-2 were purified from BL21 (DE3) bacteria transformed with pNJ420 and pNJ440, respectively, following the same protocol as previously described in Joly et al. (2016) and stored in 50 mM Tris HCl, pH 8.0, 500 mM NaCl, and 5% glycerol.