Structure prediction and homology modeling for cce4228

The amino acid sequence of cce4228 protein and SSADHs from other sources were aligned using ClusltalW method in MEGA 6.0 software. The tertiary structure of cce4228 protein was simulated with the ternary complex of SSADH from Synechococcus sp. PCC7002 with NADP⁺ and SSA (PDB ID: 3VZ3) as template by Swiss-Model [27] and depicted by Pymol 1.503 software [28]. The SSADH protein in 3VZ3 is that of the non-catalytic Cys262Ala variant [19]. The online SAVES v5.0 server (https://servicesn.mbi.ucla.edu/SAVES/) was used to evaluate the quality of simulated three-dimensional structure of cce4228 protein.