ChIP analysis

Chromatin immunoprecipitation assays were carried out using the Agarose ChIP kit from Thermo Scientific, according to the manufacturer’s guidelines. Briefly, cell samples were crosslinked by 1% formaldehyde for 10 min, and the reaction was stopped by the addition of glycine to a 125 mM final concentration. The fixed cells were lysed in SDS buffer, and the chromatin was fragmented by microccocal nuclease digestion. The sheared chromatin was incubated with antibodies against DOT1L (Bethyl, A300-954A; dilution 1:50), GCN5 (Santa Cruz, sc-20698; dilution 1:20), H3K4me3 (Abcam, ab8580; dilution 1:100), H3K9ac (Abcam, Ab4441; dilution 1:125), H3K79me2 (Abcam, Ab3594; dilution 1:100), EP300 (Abcam, ab14984, clone 3G230/NM-11), PPARGC1A (Santa Cruz, sc-13067; dilution 1:20), SIRT1 (Abcam, Ab12193; dilution 1:100) and recovered by binding to protein A/G agarose. Eluted DNA fragments were used directly for qPCR. Primers used for ChIP-qPCR analysis are listed in Supplementary Table 4.