Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Request a Protocol
Ask a
question
Favorite
Lysate was suspended at 10 mg/ml in 0.15 M Tris-HCl, pH 8, to which NaOH was added to 0.16 M. The sample was then incubated at 37°C for 2 h. TFA (10% solution) was added to a final concentration of 1% to stop the reaction. Samples were dried on a SpeedVac, and the dried material was suspended in water.
Copyright and License information: ©2016 Xia et al.
This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
