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Hierarchical and K-means clustering of SP21 and CX229 expression
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Percent area stained for SP21 and CX229 dual-stained FFPE tissue from the NHS1 and YWBCTP cohorts were separately subjected to hierarchical and K-means clustering, and optimal cluster numbers were obtained. Hierarchical clustering was performed using R studio software. For K-means clustering, the lowest expression group was identified as the distribution containing the negative stained group above which all values would be considered positive65,66.
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