Baseline and follow-up blood samples were collected using standard venipuncture procedure performed by skilled phlebotomists, centrifuged, aliquoted and stored at − 80 °C in a biobank at Lillebaelt Hospital, Vejle, Denmark.
Baseline serum samples were obtained 1–42 days (mean 7 days) prior to primary surgery for breast cancer. A postoperative sample was obtained between 14 and 30 days after primary surgery. Serum samples were subsequently obtained at standard clinical control visits. In the present study we included follow-up samples from patients who experienced metastatic recurrence. These samples were obtained within three months prior to a diagnosis of a systemic recurrence of breast cancer.
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