4.1. Seed Material

The following wheat seeds were studied: winter varieties of Triticum aestivum cv. Hondia (H), Wilejka (W), STH, Opcja (O), Tybalt (T), and Euforia (E)and Triticum spelta cv. Rokosz (R) and Schwabencorn (Sch) (Figure 8). The seed material was produced by Plant Breeding Strzelce Sp. z o.o. Group IHAR. Prior to DNA isolation, the wheat seeds were surface sterilized following the protocol proposed by Kuźniar et al. [5] and left for overnight imbibition in sterile water at 4 °C. Then, the embryos were carefully and aseptically isolated with the use of a preparative needle and a binocular microscope. The endosperm was sterilized according to the protocol developed by Kuźniar et al. [5].