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15-mer BRAF wild-type and mutated BRAFV600E peptides were synthesized by the Proteomics Resource Center at The Rockefeller University, as previously described.28 The mutated residue was placed at position +7 of the 15 amino acid sequence. All peptides were reconstituted in DMSO. A pool of peptides derived from cytomegalovirus, Epstein-Barr virus and influenza virus (CEF; AnaSpec Inc) were used as a positive control.
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