RNA isolation and gene expression analysis

Total RNA was extracted from various tissues of the transgenic lines or WT plants using the TRIzol reagent (Invitrogen). Complementary DNAs were synthesized using an M‐MLV reverse transcriptase kit (Toyobo). The LightCycler480 SYBR Green I Master Kit (Roche) was used for the qPCR analysis. Three biological replicates from each genotype were analysed to test for statistically significant differences. The actin gene ({"type":"entrez-nucleotide","attrs":{"text":"BT013524","term_id":"47104939","term_text":"BT013524"}}BT013524) was used as an internal control. The primer sequences used in real‐time PCR are listed in Table S1.