Sequence analysis

Protein sequences corresponding to full ORF orthologs of C. elegans POD-2 were collected from wormbase parasite (parasite.wormbase.org). Domain structures of ACC of C. elegans and H. schachtii were illustrated using DOG 2.0³⁴. Sequences were aligned using ClustalW. The evolutionary history was inferred using the Neighbor-Joining method³⁵. The optimal tree with the sum of branch length = 4.54642297 is shown. The percentage of replicate trees in which the associated taxa clustered together in the bootstrap test (1,000 replicates) are shown next to the branches³⁶. The tree is drawn to scale, with branch lengths in the same units as those of the evolutionary distances used to infer the phylogenetic tree. The evolutionary distances were computed using the p-distance method³⁷ and are in the units of the number of amino acid differences per site. The analysis involved 60 amino acid sequences. All positions containing gaps and missing data were eliminated. There were a total of 234 positions in the final dataset. Evolutionary analyses were conducted in MEGA5³⁸.