Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

Plants material

LS Lee Sanchez
SP Shankar Pant
KM Kranthi Mandadi
DK Dmitry Kurouski
request Request a Protocol
ask Ask a question
Favorite

Twelve leaf samples (three leaves from a branch per tree) were collected from four trees in greenhouse-grown healthy (GHH), in field-grown healthy (IFH), nutrient deficient (ND) and HLB early (non-symptomatic) of orange (Citrus sinensis, Valencia) and grapefruit (Citrus paradasi, Rio red) from Weslaco, Texas. Leaf samples were collected in Ziploc bags and immediately brought to laboratory for RS. After RS, the leaf samples were stored in −80 °C for DNA isolation. Four orange trees of IFH surrounded by CLas positive oranges trees were selected to monitor the growth of CLas over time. Three leaves were collected from the same branch of each tree to quantify CLas titer using qPCR and RS for every three months over 6 months, starting in January 2019.

Copyright and License information: The Author(s) ©2020
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A