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Primer design and evaluation.

KR Krishna Rao
AS Anna Seekatz
CB Christine Bassis
YS Yuang Sun
EM Emily Mantlo
MB Michael A. Bachman
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Candidate primer and probe assays were optimized using Visual OMP software (DNA Software, Ann Arbor, MI), which uses secondary structures and thermodynamics of DNA hybridization to predict the melting temperatures of primers and probes compared to those of unintended dimerization products and calculate the expected efficiency of the PCR. Primer and probe coverage and specificity were evaluated using the Ribosomal Database Project’s Probe Match (RDP database release 11, update 5) (2).

Copyright and License information:  ©2020 Rao et al.
This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.

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