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Freeze-Drying of Nanoparticles

GP Gopal Patel
NT Neeraj Singh Thakur
VK Varun Kushwah
MP Mahesh D. Patil
SN Shivraj Hariram Nile
SJ Sanyog Jain
UB Uttam Chand Banerjee
GK Guoyin Kai
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Further, the synthesized liposomes were freeze-dried in order to enhance their storage stability using a lyophilizer (Wizard 2.0, Virtis, United States) (Garg et al., 2015). Initially, 5% concentration of mannitol, trehelose and sucrose were screened as the cryoprotective agents for better stabilization of liposomes. Among these, mannitol was found to be best cryoprotective agent. In the subsequent experiment, different concentrations (2.5–10%) of mannitol were used to find out the optimum value of mannitol for maximum stability of liposome in minimum concentration of mannitiol.

Copyright and License information:  ©2020 Patel, Thakur, Kushwah, Patil, Nile, Jain, Banerjee and Kai.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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