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Growth for live-cell microscopy.

SS Stefanie Spriewald
ES Eva Stadler
BH Burkhard A. Hense
PM Philipp C. Münch
AM Alice C. McHardy
AW Anna S. Weiss
NO Nancy Obeng
JM Johannes Müller
BS Bärbel Stecher
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Initially, bacteria were streaked on LB agar plates containing appropriate antibiotics and incubated o.n. at 37°C. A single colony was used to inoculate 3 ml LB in test tubes (Cultube sterile culture tubes, tubes with cap, polystyrene; 17 mm × 95 mm H) and incubated for 12 h at 37°C in a rotor wheel. This o.n. culture was used to inoculate 2 ml LB medium (ratio 1:20). The subculture was incubated for 2 h in a rotor wheel at 37°C. Next, a bacterial sample of this subculture was taken and diluted in 1× phosphate-buffered saline (PBS) to achieve the concentration of 106 bacterial cells/ml.

Copyright and License information:  ©2020 Spriewald et al.
This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.

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