Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

Expression plasmids

ZG Zane A Gibbs
LR Luis C Reza
CC Chun-Chun Cheng
JW Jill M Westcott
KM Kathleen McGlynn
AW Angelique W Whitehurst
request Request a Protocol
ask Ask a question
Favorite

ZNF165 and ZNF446 cDNA was purchased in pDONR223 vectors from the DNASU Plasmid Repository (Tempe, AZ) and cloned into pLX302-V5 or pLX304-V5 lentiviral vectors, respectively, using the Gateway Cloning system (Thermo Fisher Scientific). psPAX2 and pMD2.G were gifts from Didier Trono (Addgene plasmids #12260 and #12259). SMAD3, SMAD4, and TRIM27 cDNA was obtained in pENTR221 vectors from the UT Southwestern (UTSW) McDermott Center for Human Genetics and subcloned into pCMV-HA or pCMV-myc (Clontech, Mountain View, CA) between SalI and NotI restriction sites. pCMV6-ZNF165-myc was obtained from Origene (Rockville, MD) (RC205600). The SCAN domain of pCMV6-ZNF165-myc was deleted using overlap extension PCR and verified by sequencing and western blot (primers listed in Supplementary file 4).

Copyright and License information:  ©2020, Gibbs et al
This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A