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2.6. Assessment of the Frequency of Abnormalities in the First and the Second Meiotic Divisions

MS Maria Shishova
RP Roman Puzanskiy
OG Olga Gavrilova
SK Shamuhommed Kurbanniazov
KD Kirill Demchenko
VY Vladislav Yemelyanov
GP Galina Pendinen
AS Alexey Shavarda
TG Tatjana Gavrilenko
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Flower buds of male-sterile and male-fertile potato genotypes were fixed in ethanol-acetic acid solution (3:1 v/v) in room temperature over 24 h. The fixated material was stored in 70% ethanol until analysis. Appropriate-sized anthers were stained by 2% acetoorcein and the squashed slides were prepared. The frequencies (%) of irregularities were studied at different phases of meiosis by light microscopy.

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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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