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Statistical analyses were carried out using SPSS software, version 17.0 (SPSS Inc., USA). Student's t test was used for statistical comparison between groups of asexual parasitemia, gametocytes, sex ratio, exflagellation, and ookinete numbers. The Mann-Whitney U test was employed to analyze oocyst density (oocyst number per midgut), while Fisher's exact test was used to analyze infection prevalence. All data are presented as the mean ± standard error of the mean (SEM). A P value of <0.05 was considered statistically significant.
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