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RNAi transfection

ST Sheng-Feng Tsai
MT Michael Tao
LH Li-Ing Ho
TC Tzyy-Wen Chiou
SL Shinn-Zong Lin
HS Hong-Lin Su
HH Horng-Jyh Harn
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The DDIT3 siRNA targeting the sequence AGA-GGC-AUA-CCA-AGA-UCC-A and NAG-1 siRNA targeting the sequence GAC-UCC-AGA-UUC-CGA-GAG-U were synthesized (Ambion, Austin, TX, USA). Cells at 50–60% confluence were transfected with the transfection reagent lipofectamine 2000 in accordance with the manufacturer's protocol, with DDIT3 or NAG-1 siRNA (Ambion) at concentrations of 5–20 nM for 48 hours. The transfected medium was removed, and the cells were treated with K8 or a vehicle for up to 48 hours. After incubation, RNA was isolated for RT-PCR, and the cells were collected for apoptosis analysis.

Copyright and License information:  ©2017 Tsai et al.
This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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