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Apoptosis assay

CM Callie R. Merry
SM Sarah McMahon
MF Megan E. Forrest
CB Cynthia F. Bartels
AS Alina Saiakhova
CB Courtney A. Bartel
PS Peter C. Scacheri
CT Cheryl L. Thompson
MJ Mark W. Jackson
LH Lyndsay N. Harris
AK Ahmad M. Khalil
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Cells were plated at 5000 cells/well in a 96-well plate. After 5 hours, the media was replaced and trastuzumab (10 μg/ml) was added. After 48 hours, Caspase-Glo® 3/7 Assay (Promega, G8091) reagent was added to the wells. The plate was incubated for 1 hr at room temperature and the luminescence was read by spectrophotometer. The luminescence reading was normalized to the mock treatment of each cell line to calculate relative apoptosis.

Copyright and License information:  ©2016 Merry et al.
This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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