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Detection of IL-2 production in Jurkat T cells
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HCT116 cells and Jurkat T cells were co-cultured in the presence or absence of additives (10 μg/ml anti-PD-1 mAb (eBioscience #16–9989) or 250 μM peptide Ar5Y_4) in complete RPMI-1640 medium for 24 hours. Jurkat T cells alone were used as the reference. Supernatants were harvested and assessed for IL-2 by ELISA (Thermo scientific #EH2IL2).
Copyright and License information: ©2016 Li et al.
This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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