2.18. Cell Cycle Analysis

The HEK-293T cells were fixed with 70% ethanol in PBS at 4 °C overnight. After washing with PBS three times, the cells were incubated with 100 μg/mL RNase A in PBS for 20 min. at 37 °C and then stained with 50 μg/mL propidium iodide. The percentages of cells in the G₀/G₁, S, and G₂/M phases were determined by FACSCalibur (BD Biosciences, San Jose, CA, USA) and then analyzed with Flowjo 7.6 (Treestar Inc., Ashland, OR, USA).